Literature summary extracted from

Bak, S.; Olsen, C.E.; Halkier, B.A.; Moller, B.L.
Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis (2000), Plant Physiol., 123, 1437-1448.

Application

EC Number	Application	Comment	Organism
1.14.14.36	agriculture	simultaneous expression of the two multifunctional Sorghum cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum) and Arabidopsis leads to cyanogenic plants. In transgenic plants expressing CYP79A1 as well as CYP71E1, the activity of CYP79A1 is higher than that of CYP71E1, resulting in the accumulation of several 4-hydroxyphenylacetaldoxime-derived products in the addition to those derived from 4-hydroxymandelonitrile. Transgenic tobacco and Arabidopsis plants expressing only CYP79A1 accumulate the same 4-hydroxyphenylacetaldoxime-derived products as transgenic plants expressing both sorghum cytochrome P450 enzymes. The transgenic CYP79A1 Arabidopsis plants accumulate large amounts of 4-hydroxybenzyl glucosinolate	Sorghum bicolor
1.14.14.37	agriculture	simultaneous expression of the two multifunctional sorghum cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco and Arabidopsis leads to cyanogenic plants. In transgenic plants expressing CYP79A1 as well as CYP71E1, the activity of CYP79A1 is higher than that of CYP71E1, resulting in the accumulation of several 4-hydroxyphenylacetaldoxime-derived products in the addition to those derived from 4-hydroxymandelonitrile. In transgenic Arabidopsis expressing CYP71E1, this enzyme and the enzymes of the pre-existing glucosinolate pathway compete for the 4-hydroxyphenylacetaldoxime as substrate, resulting in the formation of small amounts of 4-hydroxybenzylglucosinolate	Sorghum bicolor

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.36	Sorghum bicolor	Q43135	-	-
1.14.14.37	Sorghum bicolor	O48958	-	-

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Release 2023.1 (January 2023)